bijgh at zeus.bris.ac.uk
Tue Feb 25 12:42:06 EST 1997
Thanks to everybody for the help on peroxidase/phosphatases. I thought
I'd try you out on my next problem!
I've just done a titration of secondary antibody against my antigen, to
characterise the non-specific binding. When I added my colour substrate
(TMB in DMSO/citrate buffer) some of the wells that contained no
peroxidase conjugate gave a much larger colour change than the general
background. Is this because I have somehow contaminated these wells with
secondary antibody/conjugate, or is there another explanation?
Jared Head at the Department of Biochemistry, University of Bristol
"A computer lets you make more mistakes faster than any invention in human
history - with the possible exceptions of handguns and tequila."
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