to make afinity colums

Kiley R. Prilliman kprillim at
Wed Feb 26 20:20:45 EST 1997

(my two-cents)...

I use the Pharmacia CNBr-activated Sepharose 4B on a large scale (~100mL
bed volume columns) for antibody coupling, and the protocol is VERY EASY
and the columns appear to hold up forever!  One column has been in
almost daily use without any noticeable reduction in capacity for about
a year now, and I plan to couple at least two more within the next

Kiley R. Prilliman
Department of Microbiology & Immunology
University of Oklahoma Health Sciences Center

phone: 405-271-1203
  fax: 405-271-3117

More information about the Immuno mailing list