I've got a home-made Elisa going, and for logistic reasons I
would like to prepare (from powder) the TMB substrate in
concentrated liquid form or ideally in a ready to use form
(OK, without the H2O2). However, my liquid preparations of
TMB always die on me after a short time (loss of activity,
precipitation, colour change etc). What buffer or additive
should I be using?
Thanks
Peter Taylor
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