T cell proliferation

Ashok M S lambu at biochem.iisc.ernet.in
Thu Jan 9 01:47:16 EST 1997

Hi,        i am tryingto look for antigen specfic t cell proliferation
    but my protein get solubilised in 8M urea or guanidine hydrochloride.
I have tried electroelution, dialysized aganist 30-50% glycerol as my
protein doesnot ppt, yet i see no proliferation. I have also tried 
using inactivated virus as anitgen, again there was no proliferation.
can you help me.

thank you

dept of biochemistry
indian institute of science
bangalore 12
e-mail lambu at biochem.iisc.ernet.in

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