Life of immunostained slides?
Ken Frauwirth BioKen
frauwirt at bacillus.Berkeley.EDU
Sat Jan 11 16:08:48 EST 1997
In article <5b8if4$un at ccshst05.cs.uoguelph.ca>,
Jennifer V Robertson <jvrobert at uoguelph.ca> wrote:
>How long will the flourescence last on slides stained with FITC and
>TRITC conjugated antibodies? The slides are kept in sealed slide
>containers in the fridge.
While I am sure that this depends on the preservative/mounting medium used,
I have slides stained with FITC-conjugated antibodies that still have strong
fluorescence after over a year in a standard (not airtight) slide box at room
temp. Some cover slips were mounted with Flourosave (Calbiochem), others
with Cytoseal 60 (Stephens Scientific, available through Fisher).
Ken Frauwirth (MiSTie #33025) _ _
frauwirt at mendel.berkeley.edu |_) * |/ (_ |\ |
http://www.ocf.berkeley.edu/~frauwirt/ |_) | () |\ (_ | \|
DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs
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