How to make gene knockout model ?

[sunkdym at public.xa.sn.cn]

Hi:

Our lab had just cloned a novel molecule, which was mainly expressed on 
activated T cell and platelet. Now we are trying to find its homoeosis in mouse. 
As I know, gene-knockout model is the best method to investigate its functions.
I had tried to search for the detailed method and material of gene-knockout, but 
I could not find. I am very appreciated if anyone could e-mail me the protocol 
or some references about it. How long and how much did it need for construction 
of gene-knockout model? 
I am anxiously waiting for your reply. I hope I have not trouble you too much. 
Thank you very much. Any advice or comments are welcomed.
e-mail£º "sunkdym at puclib.xa.sn.cn"