psantos at gemini.ci.uc.pt
Thu Jan 23 15:21:57 EST 1997
Yiu-Loon Chui wrote:
> Can anyone out there kindly update me on the current advance in HLA
> typing, especially for HLA-A, B and C? Is serotyping still the main
> methodology? How about typing by PCR? Are there PCR primers available?
> How reliable are they?
> Yiu-Loon Chui
> Associate Professor
I'm performing HLA Class I (ABC) DNA typing by ARMS-PCR SSP and
Sure I find those techniques reliable.
There several papers on it, start by this one:
Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4,
DRB5, & DQB1 by PCR with 144 primer mixes utilising sequence-specific
M Bunce et al.
Tissue Antigens 1995, 46:355-367.
Feel free to ask for help.
Centro de Histocompatibilidade do Centro - LUSOTRANSPLANTE
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