HLA typing

[Paulo Santos]

Yiu-Loon Chui wrote:
> 
> Can anyone out there kindly update me on the current advance in HLA
> typing, especially for HLA-A, B and C? Is serotyping still the main
> methodology? How about typing by PCR? Are there PCR primers available?
> How reliable are they?
> 
> Thanks!
> 
> Yiu-Loon Chui
> Associate Professor

I'm performing HLA Class I (ABC) DNA typing by ARMS-PCR SSP and
PCR-SSOP.
Sure I find those techniques reliable. 
There several papers on it, start by this one:

Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4,
DRB5, & DQB1 by PCR with 144 primer mixes utilising sequence-specific
primers (PCR-SSP)

M Bunce et al. 
Tissue Antigens 1995, 46:355-367.

Feel free to ask for help.


-- 
Paulo Santos
Centro de Histocompatibilidade do Centro - LUSOTRANSPLANTE
Faculdade de Medicina de Coimbra           Fax       +351-39-33674
3º piso  Apartado 3004                     Telephone +351-39-33693
P-3049 COIMBRA Codex  PORTUGAL                       +351-39-20527