In article <K_Vora-3006971718400001 at manser3.jci.tju.edu>,
K_Vora at lac.jci.tju.edu (Kalpit A. Vora) wrote:
>Dear Readers,
>I am trying to stain mouse splenic cryosection with an polyclonal antisera
>raised in rabbits. I am having a terrible background staining problem. I
>do block with buffer containing 5% normal mouse serum(NMS) and 5% normal
>goat serum(NGS). Also all dilution of my primary and secondary antibodies
>are done in the above buffer containing NMS and NGS. Any pointers to
>improve my staining and decrease my background will be very helpful and
>thankyou in advance for the same. My e-mail address is as follows:
>kvora at lac.jci.tju.edu.>Kalpit A Vora
It seems to me that blocking with pre-immune rabbit serum would make more
sense, but I don't actually have much experience with polyclonal sera.
