Hi,
Has anyone had any experience staining Haemonchus contortus (blood-sucking
parasitic nematode of sheep) sections? Thus far, I have found them to have
endogenous peroxidase activity, autofluorescence (which limits the use of FITC
labelled antibodies to an extent) and endogenous avidin-binding activity
(which interferes with streptavidin-biotin labelled systems). Now, after
tackling the avidin-binding problem successfully, I find that the secondary
antibody (goat anti-mouse) also binds directly to the worm gut - blocking with
various agents including gelatin, skim milk powder and normal serum has failed
to quench the non-specific signal.
I have been unable to find anything in the literature re staining of
Haemonchus intestine with any antibody systems- has anyone had any success in
this area?
Thanks in advance,
Sharron
