Need marker for monocyte activation

Alan Lackey bulldogg at mindspring.com
Fri Jul 11 07:52:37 EST 1997


Some suggestions, although not restricted to monocytes alone, would
	CD69 - 	an early activation marker in lymphocytes & monocytes
	CD54 - 	expressed weakly on resting lymphs/monos and
		moderately on activated lymphs/monos

Use of CD45 & CD14 with the above antibodies in a three or four color
setup will allow for specific gating on monocytes only.

Alan Lackey
Cytometry Associates, Inc.
bulldogg at mindspring.com
alackey at cytometry.com

Andrew Louka <bb95asl at brunel.ac.uk> wrote:

>I urgently need a monocyte cell membrane marker which is upregulated 
>during monocyte activation for use in flow cytometric studies.  Ideally, 
>the marker would be specific to monocytes.
>Any tips?
>url     : http://www.brunel.ac.uk:8080/~bb95asl/                      
>finger  : bb95asl at venus.brunel.ac.uk

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