help with fixing suspension cell for staining

[Alan Lackey]

Changhong:

Have you tried coating the plate or dish with poly-l-lysine?

Alan Lackey
Cytometry Associates, Inc.
bulldogg at mindspring.com
alackey at cytometry.com

Changhong Dai <ak9 at omni.cc.purdue.edu> wrote:

>
>Hi, I am interested in methods for fixing suspension cell(say U937) for
>lacZ staining(how to make the suspension cell stick to the culture dish
>or glass for staining). Would anyone kindly share any ideas about how to
>do it?
>
>Thank you for your time.
>
>Changhong
>
>-----------------------------------
>Changhong Dai
>Tel: (765)4945401(Lab)
>Chemistry Department, P.O.Box 376
>Purdue University, West Lafayette, IN 47906