I was wondering if I someone might be able to help me; I am trying to
find a Vbeta chain in BALB/c that is eliminated upon negative selection in
the thymus. Preferably this would also be one that is present in
easily detectable numbers _before_ negative selection, too. I could also
use either CBA/caJ or C57Bl/6 if anyone knows of a scheme I could use in
these mice instead.
Also, one more request for help. I tried the above experiment with
Vbeta17a (based on something I read in Abbas), only to discover that
BALB/c has kind of a peculiar Vbeta17 gene (deleted? Vbeta17b?). Can
anyone clarify this matter for me - does BALB/c express a Vbeta17 gene, or
is this gene non-functional? A reference and/or a quick explanation
would be greatly appreciated.
Thanks in advance for your help,
University of Georgia
Department of Cellular Biology
jreese at cellmate.cb.uga.edu
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