help with fixing suspension cell for staining

Guy Hermans ghermans at luc.ac.be
Mon Jul 14 02:53:52 EST 1997

In article <33c6d294.1911009 at news.mindspring.com>,
bulldogg at mindspring.com! wrote:

> Changhong:
> Have you tried coating the plate or dish with poly-l-lysine?

> >
> >Hi, I am interested in methods for fixing suspension cell(say U937) for
> >lacZ staining(how to make the suspension cell stick to the culture dish
> >or glass for staining). Would anyone kindly share any ideas about how to
> >do it?
> >
> >Thank you for your time.

Wouldn't it be simpler just to make a cytospin of the suspension cells &
than fix them on the cover slide? Actually, when using lymphocyte
suspensions, one does not have to make any specific precautions when
spinning the cells on the slide. You can even use the most common,
non-treated microscopy glasses used everywhere!


Guy Hermans, PhD student
  Ms research Unit              Immunology research group
  Dr. L. Willems-Institute      Dept. of Physiology, LUC
  University Campus             University Campus
  B-3590 Diepenbeek             B-3590 Diepenbeek
  Belgium                       Belgium
Voice  ++32(0)11/26.92.07
Fax    ++32(0)11/26.92.09

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