You could use antibody to a framework region of MHC class II as well,
but again it's not restricted to monocytes. It also depends how early in
activation you want to look- MHC II would peak after 48 hours or so
Alan Lackey wrote:
>> Some suggestions, although not restricted to monocytes alone, would
> CD69 - an early activation marker in lymphocytes & monocytes
> CD54 - expressed weakly on resting lymphs/monos and
> moderately on activated lymphs/monos
>> Use of CD45 & CD14 with the above antibodies in a three or four color
> setup will allow for specific gating on monocytes only.
>> Alan Lackey
> Cytometry Associates, Inc.
>bulldogg at mindspring.com>alackey at cytometry.com>>> Andrew Louka <bb95asl at brunel.ac.uk> wrote:
> >I urgently need a monocyte cell membrane marker which is upregulated
> >during monocyte activation for use in flow cytometric studies. Ideally,
> >the marker would be specific to monocytes.
> >Any tips?
> >url : http://www.brunel.ac.uk:8080/~bb95asl/> >finger : bb95asl at venus.brunel.ac.uk> >O-