IUBio

Need marker for monocyte activation

Andrew Fell ahfell at netmatters.co.uk
Wed Jul 16 19:42:10 EST 1997


You could use antibody to a framework region of MHC class II as well,
but again it's not restricted to monocytes. It also depends how early in
activation you want to look- MHC II would peak after 48 hours or so
IMEx.

Andy Fell

Alan Lackey wrote:
> 
> Some suggestions, although not restricted to monocytes alone, would
> be:
>         CD69 -  an early activation marker in lymphocytes & monocytes
>         CD54 -  expressed weakly on resting lymphs/monos and
>                 moderately on activated lymphs/monos
> 
> Use of CD45 & CD14 with the above antibodies in a three or four color
> setup will allow for specific gating on monocytes only.
> 
> Alan Lackey
> Cytometry Associates, Inc.
> bulldogg at mindspring.com
> alackey at cytometry.com
> 
> 
> Andrew Louka <bb95asl at brunel.ac.uk> wrote:
> 
> >
> >I urgently need a monocyte cell membrane marker which is upregulated
> >during monocyte activation for use in flow cytometric studies.  Ideally,
> >the marker would be specific to monocytes.
> >
> >Any tips?
> >
> >Andrew
> >
> >--
> >url     : http://www.brunel.ac.uk:8080/~bb95asl/
> >finger  : bb95asl at venus.brunel.ac.uk
> >O-
> >



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