spaul at rex.uokhsc.edu wrote:
>> I am looking for an assay to estimate the amount of Hydrogen peroxide
> and superoxide produced by antigen stimulated macrophages. I tried a
> couple of the methods from papers but without any luck. I dont know
> where I am going wrong. Is anyone out there who have done assays like
> this and can share their expertise with me? Thank you very much in
> advance.
>> SPaul
SPaul
Sigma sells a kit for doing leucocyte (myeloperoxidase) peroxidase
assays. From what I understand, it is a straight-forward. Check out
the Diagnostic Kit and Reagent section of the Sigma catalog.
Eric Wadman