Recombinant nucleoprotein

Bryan J. Maloney bjm10 at c$or$ne!ll#.e&du
Fri Jul 25 12:06:27 EST 1997

In article <Pine.GSO.3.95.970723160607.29483A-100000 at dilbert.ucdavis.edu>,
David Verhoeven <ez052183 at mailbox.ucdavis.edu> wrote:

> Does anyone know of a good method for obtaining a lysate of an E coli
> expression system that is recombinant for a viral nucleoprotein.  I am
> looking for something that will be gentle enough on the nucleoprotein to
> dectect it on Elisa as well as a western blot.  I appreciate any help you
> can give.

Lysozyme treatment followed by sonication (both on ice, if possible).

Sonication must be in short bursts or with a cup-horn to avoid sample heating.

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