Homemade Chemiluminescent Protocols?
amcbride at bilbo.bio.purdue.edu
Mon Jun 2 14:01:52 EST 1997
David Aldridge wrote:
> In article <338EFCA7.41C6 at risotto.mit.edu>, Thomas Cameron
> <cameron at risotto.mit.edu> wrote:
> > Company kits end up costing $3-5 per western blot which is 90% of the
> > cost of the whole procedure.
> > People must have some good homemade HRP (and AlkPhos) Chemiluminescent
> > Detection protocols or references?
> > Could you send me one or point me to it? Thanks.
> *Luminol 4mg/ml in DMSO 1ml
> *p-iodophenol 1mg/ml in DMSO 1ml
> 1M Tris.HCL pH 7.5 0.6ml
> H2O2 (30%) 5 microL
> H2O 7.5ml
> *Store at -20
> Mix this stuff up fresh a few minutes before use.
> Works for me!
Where can I buy the luminol and the p-iodophenol from
Thanks in advance
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