Homemade Chemiluminescent Protocols?

Ali McBride amcbride at bilbo.bio.purdue.edu
Mon Jun 2 14:01:52 EST 1997


David Aldridge wrote:
> 
> In article <338EFCA7.41C6 at risotto.mit.edu>, Thomas Cameron
> <cameron at risotto.mit.edu> wrote:
> 
> > Company kits end up costing $3-5 per western blot which is 90% of the
> > cost of the whole procedure.
> >
> > People must have some good homemade HRP (and AlkPhos) Chemiluminescent
> > Detection protocols or references?
> >
> > Could you send me one or point me to it?  Thanks.
> 
> *Luminol 4mg/ml in DMSO         1ml
> *p-iodophenol 1mg/ml in DMSO    1ml
> 1M Tris.HCL pH 7.5              0.6ml
> H2O2 (30%)                      5 microL
> H2O                             7.5ml
> 
> *Store at -20
> Mix this stuff up fresh a few minutes before use.
> Works for me!
Where can I buy the luminol and the p-iodophenol from
Thanks in advance
Ali



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