Homemade Chemiluminescent Protocols?

Dr E. Buxbaum EB15 at le.ac.uk
Wed Jun 4 12:16:40 EST 1997


hk-miami at ix.netcom.com(HK) wrote:

>Maybe it wasn't the luminol...Maybe you mixed or measured something
>incorrectly, since you said that you only tried it once.
>I buy Luminol from Sigma...it works just fine.  In a side-by-side
>test of my solutions and the ones in the  Amersham ECL kit, my
>solutions work equally as well.

I would not say things like this if I hadn't done the ovious tests. In 
general I had quite a few problems with Sigma's chemicals over the years, 
for example SDS which was insoluble in water, inactive enzyme... Looks 
like a quality control problem to me. 




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