Homemade Chemiluminescent Protocols?
Dr E. Buxbaum
EB15 at le.ac.uk
Wed Jun 4 12:16:40 EST 1997
hk-miami at ix.netcom.com(HK) wrote:
>Maybe it wasn't the luminol...Maybe you mixed or measured something
>incorrectly, since you said that you only tried it once.
>I buy Luminol from Sigma...it works just fine. In a side-by-side
>test of my solutions and the ones in the Amersham ECL kit, my
>solutions work equally as well.
I would not say things like this if I hadn't done the ovious tests. In
general I had quite a few problems with Sigma's chemicals over the years,
for example SDS which was insoluble in water, inactive enzyme... Looks
like a quality control problem to me.
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