I tried this once it was much less sensitive than super signal from
Pierce Chemicals. By the way Pierce is 1/2 the price of ECL from
Amersham, and is more sensitive. I suggest you try the homemade stuff
for yourself but make sure you do the controls the chemicals were very
cheap around $20. I found protocol in the Red Book. It is listed in
the other replies.
Good Luck
Mark
Thomas Cameron wrote:
>> Company kits end up costing $3-5 per western blot which is 90% of the
> cost of the whole procedure.
>> People must have some good homemade HRP (and AlkPhos) Chemiluminescent
> Detection protocols or references?
>> Could you send me one or point me to it? Thanks.
>> --
> Thomas Cameron
