blocking Fc receptors

Melissa Callahan mkc125 at psu.edu
Tue Jun 24 10:29:50 EST 1997


I'm trying to detect CD14 on THP-1 cells (human moncyte line) but am having 
some trouble.  I've been using human IgG to block the Fc receptors then 
following with anti-CD14 and then FITC conjugated secondary Ab.  Is there any 
reason why this should not work?  I'm kinda at a loss here so any help would 
be greatly appreciated.  
Thanks in advance-- Missy 



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