blocking Fc receptors

Maurice Jensen mlj at
Sun Jun 29 16:44:29 EST 1997

Melissa Callahan wrote:
> I'm trying to detect CD14 on THP-1 cells (human moncyte line) but am having
> some trouble.  I've been using human IgG to block the Fc receptors then
> following with anti-CD14 and then FITC conjugated secondary Ab.  Is there any
> reason why this should not work?  I'm kinda at a loss here so any help would
> be greatly appreciated.
> Thanks in advance-- Missy
I hope your Primary is not unconjugated followed by Anti Human-Fitc or
it will not work as I described in my previous response.

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