blocking Fc receptors

Maurice Jensen mlj at sanasys.com
Sun Jun 29 16:41:04 EST 1997


Melissa Callahan wrote:
> 
> I'm trying to detect CD14 on THP-1 cells (human moncyte line) but am having
> some trouble.  I've been using human IgG to block the Fc receptors then
> following with anti-CD14 and then FITC conjugated secondary Ab.  Is there any
> reason why this should not work?  I'm kinda at a loss here so any help would
> be greatly appreciated.
> Thanks in advance-- Missy
Try using whole Human serum instead of just IgG. Pre-incubate your cells
with 50% Human Serum for 10 min. at 4C then keep at least 5% Human serum
in your staining solutions. 
Good Luck



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