CD4 depletion

Hon-Man Lee hlee at BCM.TMC.EDU
Fri Mar 7 12:37:32 EST 1997

Hi, I have some questions on CD4 cell depletion in mouse.I inject GK1.5 
ascite 0.5ml i.p. on D0, D2 and D4, and then look at spleen cells for CD4 
depletion on D6. There were no CD3+CD4+ cells, but the number of CD3+CD4- 
cells did not equal that of CD3+CD8+ cells, i.e. percent CD3+CD4- cells equal 
the sum of CD3+CD8+ and CD3+CD8- cells. When I use the same method to 
deplete CD8 cells by 2.43 ascite, I got no CD8+ cells and the number of 
CD3+CD8- cells equal that of CD3+CD4+, so CD8 cells are successfully 
depleted. My question is : do you think I got CD4 depletion in my experiment?

hon-man lee

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