What's the best way to sort T cells?

margaret koziel MD mkoziel at WEST.BIDMC.HARVARD.EDU
Tue Mar 25 09:39:21 EST 1997


I would like to perform a pilot experiment in which I sort T cells into
CD4,CD8, CD28, and possibly Cd45RO/RA.  I know that FACS sorting will
give me the best purity, but it would be fairly expensive for me to do
this.  What's your opinion regarding Ab coated magentic beads vs. the Ab
coated tissue culture flasks that are available?  I'm aiming for 90-95%
purity. 
Thanks.

Margaret Koziel
Beth Israel Deaconess Medical Center
Boston MA



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