immunofluorescence

Chunming Dong CDong at PRL.PULMONARY.UBC.CA
Wed Nov 5 14:42:39 EST 1997


Hello there:

I am reviewing a grant.  The investigator proposed to use 
immunofluorescence (IF) staining on paraffin-embedded tissue sections. 
Hematoxylin counterstaining will be used to view the nuclei.  I just 
wonder whether IF can be used on paraffin sections or not and whether
fluorescence microscope will be able able to see the nuclei 
counterstained by hematoxylin or not.  Any opinions will highly 
appreciated.

Chunming



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