Hello there:
I am reviewing a grant. The investigator proposed to use
immunofluorescence (IF) staining on paraffin-embedded tissue sections.
Hematoxylin counterstaining will be used to view the nuclei. I just
wonder whether IF can be used on paraffin sections or not and whether
fluorescence microscope will be able able to see the nuclei
counterstained by hematoxylin or not. Any opinions will highly
appreciated.
Chunming
