Attaching cells to Biosilon beads

Christopher Hatton cd.hatton26 at bigfoot.com
Thu Nov 27 12:05:48 EST 1997


Russell Mortishire-Smith wrote:
> 
> I'm a postgraduate student doing a PhD in association with Merck, and
> I am using Biosilon beads from Nunc to immobilize LTK- cells
> for in vitro experiments. The cells grow fine in Dulbecco's medium,
> but when the medium is changed to simple buffer (for the NMR expt)
> containing just NaCl, KCl, MgSO4, phosphate, HEPES &c the cells
> rapidly fall off the beads. I can't use Dulbecco's for the
> experiments.
> 
> Does anyone have any ideas as to why this should be, or how to get
> around it.

Hi Ailish/Russell,

the 1st thing that springs to mind is a lack of Ca2+ ions in the new
buffer, 1-1.5mM CaCl2 should be enough also is the pH always about 7.4.
I've never worked with Biosilon beads, but some cells don't always like
non plastic surfaces (charge problems) if there is no Calcium or other
free proteins swimming around.

Well this was my experience with RBL-h2 cells, and C6-glioma cells.

yours 

Christoph.
BL-Institut für Neurobiologie
Magdeburg
Germany.


 




> Thanks!
> 

-- 
Life......is well....just one of them things that most people find hard
to avoid.
(Sir Marcus Browning MP sketch at Belfast city hall 1980's-from that
rather famous Rowan man)



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