complement

[Simon Easterbrook-Smith]

The classical complement pathway is dependent on both Ca2+ and Mg2+ and the
alternative pathway is Mg2+ dependent, so EDTA will reversibly block both.



In article <6113e4$6hm at peabody.colorado.edu>, maniero at stripe.Colorado.EDU
(MANIERO GREGORY) wrote:

|| Could anyone tell me if EDTA fixes complement?  I would like to be able to
|| look at cells and complement activity in the same blood sample.  I know
|| heparin won't work but was wondering about EDTA or citrate.
|| 
|| Thanks for your help
|| 
|| -- 
|| Greg Maniero                            | Stars move with silent sounds.       

|| University of Colorado                  | The universe is calm.
|| Dept of Environmental, Population,      | Leaning on a staff, I climb
|| and Organismic Biology                  | up to the terrace.

Simon
-- 
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Dr Simon B Easterbrook-Smith    Voice: (+) 612 9351 3905
Department of Biochemistry      FAX: (+) 612 9351 4726
University of Sydney            Email: sbe at biochem.usyd.edu.au
Sydney NSW 2006
AUSTRALIA
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