Antibody level determination

James Good jsgREMOVETHIS at mole.bio.cam.ac.uk
Sun Oct 5 03:49:41 EST 1997


Dear Reader

I'm confused.

There are several ways to determine the level of specific serum antibodies
to a particular antigen : end point titre, ug antibody per ml serum,
percentage of a reference serum, OD at a given dilution, etc. What I need
to know is this : what are the pros and cons of each method? Is one more
suitable than another for a given application? Which is better when it
comes to calculating antibody isotype ratios (ie which gives a nearest
approximation to the truth)?

Thanks for any help

James



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