Does anyone have a protocol for detecting INTRACELLULAR antigen by using flow
cytometry?
I tried using phosphate-citrate buffer to increase membrane permibility and
let antibody pass membrane, but I failed.
If you get one protocol for this, please send me a copy. Thank you.
Ying F. Yang (Mr.)
Research Associate
2015 Linden Dr. West Madison WI 53706
Fax: 608-265-8020
Tel: 608-265-3269
E-mail: YangY at svm.vetmed.wisc.edu