flow cytometry protocol

[YFYang]

Does anyone have a protocol for detecting INTRACELLULAR antigen by using flow
 cytometry?

 I tried using phosphate-citrate buffer to increase membrane permibility and
 let antibody pass membrane, but I failed.

If you get one protocol for this, please send me a copy. Thank you.
Ying F. Yang (Mr.)
Research Associate
2015 Linden Dr. West Madison WI 53706
Fax: 608-265-8020
Tel: 608-265-3269
E-mail: YangY at svm.vetmed.wisc.edu