flow cytometry protocol

YFYang yfyang at aol.com
Mon Oct 13 15:50:43 EST 1997


Does anyone have a protocol for detecting INTRACELLULAR antigen by using flow
 cytometry?

 I tried using phosphate-citrate buffer to increase membrane permibility and
 let antibody pass membrane, but I failed.

If you get one protocol for this, please send me a copy. Thank you.
Ying F. Yang (Mr.)
Research Associate
2015 Linden Dr. West Madison WI 53706
Fax: 608-265-8020
Tel: 608-265-3269
E-mail: YangY at svm.vetmed.wisc.edu



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