Antigen-specific T cell proliferation debate

Scott ssimpson at acs.bu.edu
Thu Oct 23 13:16:47 EST 1997


Hello

I am having a debate with an colleague over the interpretation of some
results from a cellular proliferation assay using [3-H] thymidine
incorporation.  He argues that the cellular proliferation observed when
human PBMC are stimulted in vitro with an antigen is direct measurement
of antigen-specific T cell proliferation.  He argues that there is
published data supporting him but he cannot remember where he read it.
I disagreed with him stating that T cells are not the only cells that
are going to proliferate following antigenic stimulation of whole PBMC.
Which one of us is right and could you possibly provide us with any
references?

Thanks Scott




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