complement assay and islet tissue

JRingeling jringeling at aol.com
Fri Oct 24 14:11:42 EST 1997


Hi there:

I want to establish a complement assay with porcine islet cells after
 incubation with certain antibodies to produce cell lysis!

Does anyone have any protocols or hints? E.g. does it matter what species the
 complement is from, what concentration do I use etc..

Thanks in advance

John



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