Get rid of immunoprecipitate background

Mingzhong Chen mzchen at NWU.EDU
Tue Oct 28 21:27:54 EST 1997


Dear Netters,

I am doing immunopricipitation now. The antibody I used to precipitate
target protein is raised from the same spiecies from which the primary
antibody for Western Blotting raised.
So I got heavy backgroud of immunoglobin. I heard there is a method
which can diassociate the antigen for the Ag-Ab-Protein A bead complex.
If anybody know the detail procedure, please let me know.  I would be
very appreciate your help. Please E-mail me at: mzchen at nwu.edu

Thanks lots.


Mingzhong Chen
---------------
Northwestern University



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