Get rid of immunoprecipitate background

[Mingzhong Chen]

Dear Netters,

I am doing immunopricipitation now. The antibody I used to precipitate
target protein is raised from the same spiecies from which the primary
antibody for Western Blotting raised.
So I got heavy backgroud of immunoglobin. I heard there is a method
which can diassociate the antigen for the Ag-Ab-Protein A bead complex.
If anybody know the detail procedure, please let me know.  I would be
very appreciate your help. Please E-mail me at: mzchen at nwu.edu

Thanks lots.


Mingzhong Chen
---------------
Northwestern University