> Anyone out there know of a bioassay for GM-CSF activity?
> Specifically, I'm trying to determine if human or canine GM-CSF exerts a
> biologic effect in the equine system.
>> Thanks in advance for any insights/experiences/references.
There are a number of cell lines that proliferate in response to GM-CSF
(and IL-3). We use a human erythroleukemia cell line called TF-1 that
is GM-CSF dependent and can be used in bioassay. See Kitamura et al.,
J. Cell. Physiol. 140: 323-334, 1989). This line is available from
ATCC. Other lines include FDC-P1 (Dexter et al. J. Exp. Med 152:
1036-1047, 1980); BAC1 (Morgan et al., J.Cell. Physiol. 130: 420-427
(1987); NFS-60 (Hara et al. EMBO J. 11: 1875-1884,1988) TALL101
(Valtieri et al. J. Immunol. 138: 4042-4048, 1987) and the murine line
HT2 (Kupper et al. J. Immunol 138: 4288-4292, 1987) . I"m afraid I know
nothing about the responsiveness of these lines to equine or canine
GM-CSF. Hope this helps. -- D.G. Spinella