Andrew Schrader wrote:
>> I would appreciate some help in finding a source for a monoclonal
> andtibody directed against the alpha chain of the high affinity IgE
> receptor - Fc epsilon RI.
>> I have tried a number of commercial organisations without success
> (Genzyme, Calbiochem, R&D, Zymed, Biomedicals AG and Biodesign).
>> Thank you
>> Andrew
> --
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Dr Andrew P. Schrader
> Postdoctoral Fellow
> (Respiratory Research Group)
> Department of Pharmacy (A15)
> The University of Sydney NSW 2006
>> Telephone:
> Office (Rm S114): +61 2 9351 3647/8
> Laboratory (Rm N334): +61 2 9351 2670/3848
> Voice Mail/Mobile: +61 15 915 192
> Facsimile: +61 2 9351 4391
> Email: andrews at pharm.usyd.edu.au> URL: http://www.usyd.edu.au/su/pharmacology/resprg.html>> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Andrew,
Suggest you correspond with these folks.
--
Jeffrey Smoot (jsmoot at cas.org)
614-447-3600 X3198
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Chemical Abstract Services http://www.cas.org
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TI Allergen-induced recruitment of Fc.epsilon.RI+ eosinophils in human
atopic skin
AU Barata, Luis T.; Ying, Sun; Grant, J. Andrew; Humbert, Marc; Barkans,
Julia; Meng, Qui; Durham, Stephen R.; Kay, A. Barry
CS Imperial College School Medicine, National Heart Lung Institute,
London, SW3 6LY, UK
SO Eur. J. Immunol. (1997), 27(5), 1236-1241
AB Fc.epsilon.RI+ eosinophils were identifyed in cutaneous late-phase
reaction in atopic subjects biopsied at 6, 24, and 48 h after the
injection of either allergen or a diluent control. Compared to the
diluent sites, allergen-injected sites had increased nos. of
eosinophils, peaking between 6-24 h, of which approx. 20-30%
expressed mRNA for the .alpha., .beta., and .gamma. chains of
Fc.epsilon.RI, as shown by in situ hybridization. Using either a
monoclonal or a polyclonal anti-.alpha. chain antibody, the
Fc.epsilon.RI.alpha. protein also co-localized to approx. 50-80% of
eosinophils at all time points studied. A correlation was obsd.
between the nos. of Fc.epsilon.RI+ (997+)/EG2+ eosinophils and the
magnitude of the late-phase reaction. Thus, a significant proportion
of eosinophils infiltrating the site of allergen-induced allergic
tissue reactions in atopic subjects express Fc.epsilon.RI. The
findings show that high-affinity IgE receptors may play a role in
eosinophil secretory processes in vivo.
