Q: AB penetration prob. in cryostat sections

Swidbert R Ott sro21 at cam.ac.uk
Thu Apr 2 03:26:04 EST 1998


In article <6fu4bb$nnt$1 at elmo.tju.edu>, "John Smith"
<johnsmith at microsoft.com> wrote:

> You might also want to try freeze-drying the section as opposed to air
> drying. On cytospins and regular 10-15 micrometer sections the improvement
> is impressive.

Thank you for your rply. Do you mean "real" freeze-drying, or putting on
cold slide, thawing briefly, and putting the section back into the
kryostat where they will sort of freeze-dry?

-- 
Swidbert R. OTT
Dept. Zoology, Univ. Cambridge/UK



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