Cytosolic Buffer

[che]

Hello All,

Are there any available buffers (commercial or recipes will do) that mimick
the cytosolic environment.  These buffers would presumably provide similar
pH, ionic and reducing strengths as the cytosol. I am working with isolated
lysosomes, and would like to provide them with a 'homely' environment
without the interference of other organelles. 

Thank you in advance

Che Pillay
Dept. of Biochemistry
University of Natal (PMB)
Scottsville
South Africa

Pillayc at agric.unp.ac.za