immunohistochemistry question

[Christelle Faveeuw]

Hi everybody

I have stained lymph node sections from different animals with an 
antibody specific for endothelial cells. After thawing the slides out, 
they were fixed in cold acetone for 10min. Then, they were rehydrated 
with PBS and stained with the antibody. The problem was that all the 
sections came off (after the first contact with liquid). I have done 
this experiment for years and never had any problem!! I don't have any 
explanation.
I have got one spare slide for each lymph node!! This is a very 
important experiment and I don't want the sections to come off the 
slides. Has anyone got an idea? Maybe I should use another fixativ!

Thanks a lot
Christelle