anti-His antibody

Flore Bellefontaine flore.bellefontaine at fundp.ac.be
Tue Apr 21 03:35:23 EST 1998


Hi!
Did someone ever detect with an anti-his antibody a recombinant protein
purified from pet15B (Novagen)?  The recombinant prot presents a six
histidines tag at the NH3-term.
I have been using unsuccesfully the penta-His antibody from Qiagen in
ELISA, Dot Blot under native and denaturing conditions and in Western Blot
aswell. Do you have any experience using that antibody?
Any kind of help is wellcome, thank you in advance,



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