vkurup at POST.ITS.MCW.EDU
Fri Aug 7 11:31:32 EST 1998
Dear Jose, as I know to get IL-4 production from PBMC you have to
stimulate them with a pair of anti CD28 Abs. You can find out the
information through Internet trying "IL-4 and CD28" If you have problems
ask again, I will find out the citation.
| Viswanath P. Kurup : Allergy Immunology Division |
| Professor of Medicine : Medical College of Wisconsin |
| PHONE: 414-384-2000 x1459,1510,1460: Research Service 151-I |
| FAX: 414-382-5374 : V.A. Medical Center |
| EMAIL: vkurup at post.its.mcw.edu : Milwaukee, WI 53295-1000 I
I EMAIL V.A.: KURUP.VISWANATH_P at MILWAUKEE.VA.GOV I
On 7 Aug 1998, Jose Santos Alvarez wrote:
> Date: 7 Aug 1998 07:56:25 -0700
> From: Jose Santos Alvarez <alvarez at cica.es>
> To: immuno at net.bio.net
> Subject: IL-4 secretion
> I need to stimulate PBMC to produce IL-4. I tried lectins (ConA, PHA)
> and soluble anti-CD3 and anti-alfa/betaTCR, but PBMC only make IFNgamma.
> Do you know any stimulus to produce IL-4? No matters IFNgamma
> Thank you, guys.
> Jose Santos (alvarez at cica.es)
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