In article <Pine.SOL.3.96.980807112348.7786C-100000 at post.its.mcw.edu>,
Viswanath Kurup <vkurup at post.its.mcw.edu> wrote:
> Dear Jose, as I know to get IL-4 production from PBMC you have to
> stimulate them with a pair of anti CD28 Abs. You can find out the
> information through Internet trying "IL-4 and CD28" If you have problems
> ask again, I will find out the citation.
>
> >
> > I need to stimulate PBMC to produce IL-4. I tried lectins (ConA, PHA)
> > and soluble anti-CD3 and anti-alfa/betaTCR, but PBMC only make IFNgamma.
> > Do you know any stimulus to produce IL-4? No matters IFNgamma
> > production.
Why don't you try to stimulate PBMC of an atopic donor with some allergen?
Guy
--------------------------------------------
Niels Hellings, PhD student
MS research Unit Tel 0032 (0) 11/26.92.07
Dr. L. Willems-Institute Fax 0032 (0) 11/26.92.09
University Campus E-mail hellings at luc.ac.be
B-3590 Diepenbeek
Belgium
--------------------------------------------
