Q: TRANSFECTION OF PRIMARY CELLS
Yamalik at btinternet.com
Tue Aug 18 15:45:52 EST 1998
I wish to transfect a reporter plasmid into Peripheral Blood Lymphocytes
(PBLs). At present, i have no problems transfecting T-Cell lines such as
SupT1 and PM1 cells using DEAE-Dextran method, however, there is little, if
any literature that i can find on the transfection of PBLs (except by the
use of virus). So far DEAE-Dextran method has failed to give me a result
(usually acompanied by high cell death).
Does anyone have any suggestions or references that i may follow up.
Y. A. Malik
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