Megan Montfort wrote:
>> I am trying to develop some type of protocol for antigen retrieval on formalin
> fixed, paraffin embedded sections. There seem to be so many different
> approaches and solutions being used... Does anyone any have recommendations on
> favorite methods?
> Where I should start?
>> I am trying to stain for the following antibodies, which have been undetectable
> so far in
> known positive control tissues: H2-K, CD28, CD86 (B7-2), CD119 (IFNgamma
> Receptor alpha chain).
>> I'd be VERY appreciative of any direction you could give...
>> Megan Montfort
Megan, since this is a mouse-model, the first approach is to work with
fresh-frozen tissues. these will be positive if your antibodies are good, as
you say there are many antigen retrieval protocols and it best
approached empirically. If no protocol was done on your specific tissue,
than trail and error is the way to go, good luck
markH
