IUBio

Does freezing reduce the potential of T cell to respond?

Jeff Frelinger jfrelin at med.unc.edu
Wed Aug 26 12:00:13 EST 1998


They work fine but not as well as fresh.  We freeze them in 90% FCS 10%
DMSO, in a -70 freezer in a freeaing box for sort of controlled rate
freezing

>Hello,
>Could anyone out there help me decide if I could use a properly frozen
>stock of PBMCs for development of TCL or TCC later?
>Thank you so much!
>Anastas Pashov
>
>





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