In article <6s1euq$5tn$1 at fddinewz.oit.unc.edu>, "Jeff Frelinger"
<jfrelin at med.unc.edu> wrote:
> They work fine but not as well as fresh. We freeze them in 90% FCS 10%
> DMSO, in a -70 freezer in a freeaing box for sort of controlled rate
> freezing
>> >Hello,
> >Could anyone out there help me decide if I could use a properly frozen
> >stock of PBMCs for development of TCL or TCC later?
My thoughts exactly. Frozen PBMC are perfect antigen presenting cells, so
its handy to keep a stock in liquid nitrogen. But for setting up, say, a
limiting dilution experiment, I'd never use 'old' material. Whenever
possible we try to motivate the donors to come back regularly or send in
fresh blood samples when needed.
Guy
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Niels Hellings, PhD student
MS research Unit Tel 0032 (0) 11/26.92.07
Dr. L. Willems-Institute Fax 0032 (0) 11/26.92.09
University Campus E-mail hellings at luc.ac.be
B-3590 Diepenbeek
Belgium
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