affinity purification with phage antibodies

M. Pelsers Maurice.Pelsers at FYS.rulimburg.nl
Tue Dec 1 08:56:31 EST 1998


Hello dear immunology friends,

As I recently prepared phage antibodies to the membrane protein FAT (fatty 
acid translocase, also known as CD36)(Pelsers et al. Biochem J. 1999) I was 
wondering if anyone ever tried to make an affinity colum with phages. Is it 
possible to take a Cn-Br activated colum an bind phage to it so that we can 
isolate the FAT from rat hart homogenates ??

Maurice.Pelsers at fys.unimaas.nl
Maastricht University
Dept. of Physiology
Netherlands



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