B7 expression in dendritic cells

Geoffrey M Thiele gthiele at ix.netcom.com
Fri Dec 18 17:53:53 EST 1998

I have made dendritic cells by methods found in current protocols (mouse BM
cells and GM-CSF).  I want to use the cells to find the upregulation of B7
expression.  The cells appear to be immature (low class II expression) and
further subculturing does not increase this.  The cells do express CD11c a
great deal.  Any suggestions as to solving the problem of immature cells?

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