isolate monocytes

David Tak Yan Yu dtyyu at
Wed Dec 30 18:27:44 EST 1998

I am trying to isolate monocytes from human PBMC by adherence.  When I
use RPMI-10% FCS, yield is extremely poor.  When I use RPMI with no
sera, viability is low.  What media and what plates should I use?  What
is the expected viability?

David Yu

35-40 Rehabilitation Center, Rheumatology Division, UCLA, 1000 Veteran
Ave., Los Angeles, CA90095-167022, e-mail: dtyyu at, FAX:

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