isolate monocytes

David Tak Yan Yu dtyyu at ucla.edu
Wed Dec 30 18:27:44 EST 1998


I am trying to isolate monocytes from human PBMC by adherence.  When I
use RPMI-10% FCS, yield is extremely poor.  When I use RPMI with no
sera, viability is low.  What media and what plates should I use?  What
is the expected viability?

Thanks,
David Yu

--
35-40 Rehabilitation Center, Rheumatology Division, UCLA, 1000 Veteran
Ave., Los Angeles, CA90095-167022, e-mail: dtyyu at ucla.edu, FAX:
(310)-206-8606.





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