Antibody production-ascites vs. cell culture

Jay Mone' jmone at MARAUDER.MILLERSV.EDU
Thu Feb 5 10:17:19 EST 1998


Theresa,
Production of monoclonal Abs is definately more efficient by ascites 
than by tissue culture.  Hybridomas in tissue culture generally yield 
about 50ug/ml of specific antibody, while ascites yields are typically 
1-10 mg/ml.  Problems with ascites production include variability from 
mouse to mouse in total Ab yields, and the need for mice of specific 
ages.  Of course, you also need certified animal rooms.  This makes 
ascites production an expensive undertaking.  However, if the animal 
facilities are already in place, ascites will generally give you more 
antibody than tissue culture.

Jay Mone'



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