I'm trying to solve the problem of IgM instability in serum
samples. We bring in serum samples from the field often
without ice, never mind frozen, and I'm worried about loss of
specific IgM activity when the samples are put into an ELISA.
I understand that IgM is also lost on prolonged storage at 4oC.
Is the problem principally aggregation? In that case does
diluting the serum help?
Is the problem proteases? Then add anti-proteases?
J chain instability?
Basically, what is ingredient X that I can add to serum samples
to preserve IgM?
Thanks
Peter Taylor
Centro de Medicina Experimental
Instituto Venezolano de Investigaciones Cientificas
Venezuela
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