Lymphocytes

klaus decken decken at rz.uni-leipzig.de
Fri Feb 13 10:42:00 EST 1998


ERNESTO OVIDEO ORTA wrote:
> 
> Hi, I would like to know if someone can help me to find the following
> methods:
> * Non-FACS, non-magnetic beads, human B lymphocytes isolation and
>   purification.
> * Fast double immunofluorescence for lymphocytes in suspension.
> Thank you in advance,
> 
> Ernesto.

For lymphocyte isolation try the classical method of density gradient
centrifugation with a Ficoll (or Percoll) step of 1.077 g/ml.

For hints regarding your double-staining I should know more about your
antibodies, e.g. species and isotypes of primary and secondary
antibodies.

Klaus Decken
University of Leipzig, Veterinary Medicine, Immunology Unit



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